(+)-Aphidicolin
(+)-Aphidicolin is a natural tetracyclic diterpene first isolated from the fungus C. aphidicola and shown to have antiviral activity against herpes simplex.{22496} In eukaryotic cells, it is a cell-permeable, reversible inhibitor of DNA replication, specifically blocking the activity of DNA polymerases ?, ?, and ? when used at low micromolar levels.{22501,22499,22497} Aphidicolin can be used, at 3 ?M, to arrest cells in G1/S phase or to increase gene amplification frequency.{22498,22502}
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